Localization of Hydrogen Ion and Chloride Ion Fluxes in Nitella

Na and K Fluxes in Nitella clavata

Na and K influxes and effluxes, membrane potentials, and cell ion concentrations of Nitella clavata were measured as functions of external NaCl concentrations and time. It appears necessary to conclude that active K transport into the cells as well as active Na extrusion is present, although the latter is of small magnitude and possibly is explicable as exchange-diffusion. An attempt has been m...

The Relation of the Membrane Potential and Resistance of Nitella to Ion Fluxes

The Influence of H+ on the Membrane Potential and Ion Fluxes of Nitella

The resting membrane potential of the Nitella cell is relatively insensitive to [K](o), but behaves like a hydrogen electrode. K(+) and Cl(-) effluxes from the cell were measured continuously, while the membrane potential was changed either by means of a negative feedback circuit or by external pH changes. The experiments indicate that P(K) and P(Cl) are independent of pH but are a function of ...

Hydrogen ion concenteration of the root media in relation to transpiration rates

Measurements of the Intracellular Ph in a Single Cell of Nitella Flexilis by Means of Micro-glass Ph Electrodes.

The use of micro-glass electrodes sensitive to the activity of selected cations has permitted a direct measurement of intracellular ionic activity , e. g., the activity of hydrogen ion in a single crab muscle fiber (CALDWELL 1954), and in a single squid giant axon (CALDWELL 1958, SPYROPOULOS 1960), the activity of sodium and potassium ion in a single squid giant axon (HINKE 1960). With a micro-...